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Dietary polyunsaturated fatty acid effects on immune cells of the porcine lung
Author(s) -
Turek J.J.,
Schoenlein I.A.,
Clark L.K.,
Van Alstine W.G.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.56.5.599
Subject(s) - biology , polyunsaturated fatty acid , docosahexaenoic acid , menhaden , eicosapentaenoic acid , lipopolysaccharide , corn oil , linoleic acid , linseed oil , immune system , medicine , food science , endocrinology , fish oil , fatty acid , biochemistry , immunology , fishery , fish <actinopterygii>
The effects of various dietary polyunsaturated fatty acids (PUFAs) on the function of immune cells of the porcine lung was studied. Groups of six pigs were fed diets containing 10.5% corn oil [CO; enriched in linoleic acid (18:2, n‐6)], linseed oil (LO; enriched in α ‐linolenic acid (18:3, n‐3)], menhaden oil (MO; enriched in eicosapentaenoic (20:5; n‐3) and docosahexaenoic (22:6; n‐3) acids], linseed + corn oil (1:1; LC), and menhaden + corn oil (1:1; MC) for 28–30 days. Basal levels of alveolar macrophage (m φ ) tumor necrosis factor‐ α (TNF‐ α ) production were higher (P < .05) for LC‐ and MC‐fed pigs than for CO‐ and LO‐fed pigs. Lipopolysaccharide (LPS)‐stimulated LC and MC m φ s produced more TNF than m φ s from pigs fed CO, LO, and MO diets. Macrophages from pigs receiving the CO and LC diets had higher (P < .05) levels of leucine aminopeptidase than mfs from the other dietary groups. Lipopolysaccharide did not increase m φ nitrite production over basal levels except in the MO diet group. However, LPS‐stimulated m φ s from the CO, MO, and LC dietary groups produced more nitrite than mfs from MC‐fed pigs. Alveolar lymphocytes from pigs receiving the MC diet produced more T cell growth factors than LO and MO m φ s. Alveolar m φ s from the different dietary groups did not differ in their capacity for non‐immune‐mediated phagocytosis of fluorescent latex beads. These results indicate that dietary PUFAs can modulate some functions of porcine alveolar immune cells and that this may prove significant for host response to respiratory disease agents. J. Leukoc. Biol. 56: 599–604; 1994.