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13‐HODE inhibits the intracellular calcium increase of activated human polymorphonuclear cells
Author(s) -
Velde Marco J.,
Engels Ferdi,
Henricks Paul A. J.,
Nijkamp Frans P.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.56.2.200
Subject(s) - leukotriene b4 , calcium , intracellular , metabolite , calcium in biology , linoleic acid , biology , leukotriene , biochemistry , microbiology and biotechnology , chemistry , immunology , inflammation , fatty acid , organic chemistry , asthma
We studied the effect of the linoleic acid metabolite 13‐hydroxyoctadecadienoic acid (13‐HODE) on the increase of the intracellular calcium concentration, [Ca 2+ ]i, induced by platelet‐activating factor (PAF), leukotriene B 4 (LTB 4 ), and formyl‐Met‐Leu‐Phe (fMLP) in human polymorphonuclear leukocytes (PMNs). 13‐HODE by itself did not change the [Ca 2+ ]i of PMNs. However, when PMNs were preincubated with 13‐HODE and subsequently stimulated with PAF, LTB 4 , or fMLP, an inhibition of the increase of the [Ca 2+ ]i was observed. The PAF‐induced calcium response was concentration‐dependently inhibited between 0.1 and 5 μ M 13‐HODE. These results suggest that 13‐HODE can affect the increase of the [Ca 2+ ]i and hence can modulate activation of PMNs. J. Leukoc. Biol . 56: 200–202; 1994.