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Analysis of human cytomegalovirus‐infected peripheral blood mononuclear cells from infants with liver dysfunction by flow cytometry and the polymerase chain reaction
Author(s) -
Numazaki Kei,
Asanuma Hideomi,
Nagata Nobuo,
Chiba Shunzo
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.56.2.187
Subject(s) - human cytomegalovirus , biology , flow cytometry , cd8 , asymptomatic , peripheral blood mononuclear cell , immunology , cytomegalovirus , virology , antigen , virus , herpesviridae , viral disease , pathology , medicine , in vitro , biochemistry
Perinatal human cytomegalovirus (HCMV) infection often involves the hepatobiliary tract, but infected individuals usually remain asymptomatic. We investigated the role of CD8 + lymphocytes in 13 infants with liver dysfunction associated with perinatal HCMV infection. In three patients more than 40% of CD8 + cells were positive for HCMV immediate early antigen (IEA) and late antigen (LA) by flow cytometry after selection of T lymphocytes subpopulations. In the other 10 infants, 20% to 30% of CD8 + cells were positive for HCMV IE A and LA. HCMV IE DNA was detected in CD8 + cells from one infant, in CD4 + cells from one infant, and in both CD4 + and CD8 + cells from three infants. HCMV infection of CD8 + cells may play an important role in the process of perinatal primary infection. J. Leukoc. Biol . 56: 187–191; 1994.