Analysis of human cytomegalovirus‐infected peripheral blood mononuclear cells from infants with liver dysfunction by flow cytometry and the polymerase chain reaction | Zendy

Numazaki Kei | Zendy; Asanuma Hideomi | Zendy; Nagata Nobuo | Zendy; Chiba Shunzo | Zendy

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Analysis of human cytomegalovirus‐infected peripheral blood mononuclear cells from infants with liver dysfunction by flow cytometry and the polymerase chain reaction

Author(s) -

Numazaki Kei,

Asanuma Hideomi,

Nagata Nobuo,

Chiba Shunzo

Publication year - 1994

Publication title -

journal of leukocyte biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.819

H-Index - 191

eISSN - 1938-3673

pISSN - 0741-5400

DOI - 10.1002/jlb.56.2.187

Subject(s) - human cytomegalovirus , biology , flow cytometry , cd8 , asymptomatic , peripheral blood mononuclear cell , immunology , cytomegalovirus , virology , antigen , virus , herpesviridae , viral disease , pathology , medicine , in vitro , biochemistry

Perinatal human cytomegalovirus (HCMV) infection often involves the hepatobiliary tract, but infected individuals usually remain asymptomatic. We investigated the role of CD8 + lymphocytes in 13 infants with liver dysfunction associated with perinatal HCMV infection. In three patients more than 40% of CD8 + cells were positive for HCMV immediate early antigen (IEA) and late antigen (LA) by flow cytometry after selection of T lymphocytes subpopulations. In the other 10 infants, 20% to 30% of CD8 + cells were positive for HCMV IE A and LA. HCMV IE DNA was detected in CD8 + cells from one infant, in CD4 + cells from one infant, and in both CD4 + and CD8 + cells from three infants. HCMV infection of CD8 + cells may play an important role in the process of perinatal primary infection. J. Leukoc. Biol . 56: 187–191; 1994.

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