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Macrophage precursor cells produce perforin and perform Yac‐1 lytic activity in response to stimulation with interleukin‐2
Author(s) -
Li Hao,
Pohler Uwe,
Strehlow Inga,
Hertig Sylvie,
Baccarini Manuela,
Emmendörffer Andreas,
Tschopp Jürg,
LohmannMatthes MarieLuise
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.56.2.117
Subject(s) - biology , lytic cycle , perforin , stimulation , macrophage , microbiology and biotechnology , interleukin , immunology , lymphokine , immune system , cytokine , neuroscience , biochemistry , in vitro , virus , cd8
Macrophage precursor cells, derived from mouse bone marrow culture with granulocyte‐macrophage colony‐stimulating factor or colony‐stimulating factor 1 (CSF‐1) as growth factor and interleukin‐2 (IL‐2) as stimulating factor, were activated by IL‐2 to exert strong cytolytic activity against Yac‐1 cells. In response to IL‐2 stimulation these bone marrow macrophage precursor cells produced perforin as lytic molecules. The purity of the precursor cells for the study was proved as homogeneous positivity for Mac‐1, NK‐1.1 and negativity for Lyt 1 and 2. The cells express CSF‐1 receptors on their surface, are able to proliferate and differentiate into typical macrophages when stimulated with CSF‐1, and are therefore members of the macrophage lineage. Perforin transcripts were identified by Northern blot analysis of IL‐2–treated macrophage precursor cells, and the presence of perforin protein in the cytoplasmic granules was demonstrated by immunhistochemical staining using a monoclonal antiperforin antibody. In addition, the biological activity of the perforin contained in the macrophage precursor's granules could be documented as calcium‐dependent lytic activity using Yac‐1 and sheep red blood cells as targets. The results presented in this paper imply the existence of a bipotent precursor cell, which can mature into a typical macrophage if CSF‐1 or phorbol 12‐myristate 13‐acetate is supplied as differentiation stimulating factor but develops into an NK/LAK cell when early activation with IL‐2 is provided. J. Leukoc. Biol . 56: 117–123; 1994.