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Human neutrophil respiratory burst response to influenza A virus occurs at an intracellular location
Author(s) -
Kazhdan Misha,
White Mitchell R.,
Tauber Alfred I.,
Hartshorn Kevan L.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.56.1.59
Subject(s) - respiratory burst , biology , intracellular , chemotaxis , influenza a virus , respiratory system , immunology , neutrophil extracellular traps , n formylmethionine leucyl phenylalanine , extracellular , syk , granulocyte , microbiology and biotechnology , neutrophile , inflammation , virus , receptor , signal transduction , biochemistry , tyrosine kinase , anatomy
We have studied in detail the in vitro interactions of influenza A viruses (IAVs) with human neutrophils to clarify why these cells become dysfunctional during IAV infection. Unosponized IAV elicited a respiratory burst response in neutrophils which, like that triggered by formylmethionyl‐leucyl‐phenylalanine (fMLP), involved mediation of signal‐transducing GTP‐binding proteins and tyrosine kinase activation. The IAV‐induced response differed from that provoked by fMLP in that H 2 O 2 was produced without concomitant O 2 ‐ release. IAV also did not cause extracellular release of granule enzymes in cytochalasin B‐treated neutrophils. Using chemiluminescence assays, the respiratory burst response to IAV was found to occur at an intracellular location. These findings may, in part, explain the anomalous nature of the respiratory burst response elicited by IAV and suggest strategies for determining the mechanism of IAV‐induced neutrophil deactivation. J . Leukoc . Biol. 56: 59–64; 1994.