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Selective Mycobacterium avium–induced production of nitric oxide by human monocyte‐derived macrophages
Author(s) -
Dumarey Claude H.,
Labrousse Valérie,
Rastogi Nalin,
Vargaftig B. Boris,
Bachelet Maria
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.56.1.36
Subject(s) - nitric oxide , microbiology and biotechnology , biology , lipopolysaccharide , mycobacterium , interferon gamma , monocyte , mycobacterium smegmatis , macrophage , nitric oxide synthase , tumor necrosis factor alpha , mycobacterium tuberculosis , virulence , cytokine , immunology , bacteria , biochemistry , in vitro , tuberculosis , medicine , pathology , genetics , gene , endocrinology
Infection with a virulent strain of Mycobacterium avium , but not with virulent Mycobacterium tuberculosis or avirulent Mycobacterium smegmatis , induced the formation of nitric oxide by human monocyte‐derived macrophages. This process was not affected by lipopolysaccharide or cytokines such as interferon‐ γ or tumor necrosis factor α . M. avium‐induced nitric oxide production was significantly decreased by N G ‐monomethyl‐l‐arginine, a potent inhibitor of nitric oxide synthase activity, without any significant enhancement of intramacrophagic mycobacterial growth. Infection with all the three mycobacterial species induced a significant activation of phospholipase A 2 activity of macrophages as evidenced by the increased release of thromboxane A 2 . Finally, nitric oxide production by human monocyte‐derived macrophages required infection with live M. avium , as neither gamma‐irradiated M. avium nor the subcellular fractions of this microorganism (cell wall, cytosol) were able to trigger nitric oxide synthesis. J. Leukoc. Biol. 56: 36–40; 1994.