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Rat CINC/gro: a novel mediator for locomotive and secretagogue activation of neutrophils in vivo
Author(s) -
Suzuki Hidekazu,
Suematsu Makoto,
Miura Soichiro,
Liu Yu Ying,
Watanabe Kazuyoshi,
Miyasaka Masayuki,
Tsurufuji Susumu,
Tsuchiya Masaharu
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.5.652
Subject(s) - secretagogue , biology , in vivo , mediator , microbiology and biotechnology , immunology , endocrinology , secretion
The effects of rat CINC/gro, a member of the interleukin‐8 family, on the endothelium‐neutrophil interaction and transendothelial macromolecular leakage were studied in rat mesenteric microcirculation. Thirty minutes after superfusion with 10 nM CINC/gro, the number of neutrophils adherent to the venular endothelium and those migrated across the venules were significantly increased with a concomitant elevation of luminol‐dependent chemiluminescence at the site of adhesion. Transendothelial macromolecular leakage as assessed by the relative length of venular wall stained with monastral blue B was also increased at 30 min after the start of CINC/gro superfusion. Pretreatments with a CD18‐directed monoclonal antibody, WT‐3 (1 mg/kg), significantly attenuated the increase in number of adherent and migrated neutrophils, the increase in luminol‐dependent chemiluminescence, and the venular macromolecular leakage after the application of CINC/gro. These data suggest that CINC/gro is a novel stimulator that evokes not only locomotive but also secretagogue activation of neutrophils via a CD18‐dependent mechanism in viva J. Leukoc. Biol. 55: 652–657; 1994.