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Metastatic competence of BW5147 T‐lymphoma cell lines is correlated with in vitro invasiveness, motility and F‐actin content
Author(s) -
Verschueren Hendrik,
Van der Taelen Imme,
Dewit Joëlle,
De Braekeleer Jos,
De Baetselier Patrick
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.4.552
Subject(s) - pseudopodia , biology , motility , actin , in vitro , cell culture , lymphoma , flow cytometry , phalloidin , cell , microbiology and biotechnology , cytoskeleton , pathology , immunology , medicine , biochemistry , genetics
The aim of our study was to investigate whether the level of actin polymerization plays a role in the motile and tissue infiltrating behavior of malignant lymphoma cells. For a panel of cell lines derived from the murine BW5147 T‐cell lymphoma, we had previously shown a correlation between experimental metastasis formation and in vitro monolayer invasion. We have analyzed the motility and the F‐actin content of six non‐metastatic, noninvasive (meta ‐ inv ‐ ) and five metastatic, invasive (meta + inv + ) variants of BW5147. Fourier analysis of cell contours was used to quantify shape changes of cells. All meta + inv + lines rapidly protruded and retracted pseudopodia, whereas only one of the six meta ‐ inv ‐ lines showed this type of motility. Flow cytometry of cells stained with fluorescein‐labeled phalloidin showed that the motile meta + inv + cell lines have a higher F‐actin content than their nonmotile meta ‐ inv ‐ counterparts. The results indicate that in lymphoma cells a high level of actin polymerization is a prerequisite for the formation of pseudopodia, which in turn are necessary for infiltration of the cells into tissues, and eventually for efficient metastasis formation. A corollary of this conclusion is that regulation of actin polymerization is a possible target for intervention aimed at moderating the spread of malignant lymphoma. J. Leukoc. Biol. 55: 552–556; 1994.