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HIV‐1 envelope protein gp120 affects phenotype and function of monocytes in vitro
Author(s) -
DürrbaumLandmann Iris,
Kaltenhäuser Erika,
Flad HansDieter,
Ernst Martin
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.4.545
Subject(s) - biology , phenotype , in vitro , human immunodeficiency virus (hiv) , function (biology) , envelope (radar) , microbiology and biotechnology , monocyte , immunology , virology , genetics , gene , telecommunications , radar , computer science
We investigated the effect of human immunodeficiency virus type 1 (HIV‐1) recombinant gp120 (rec.gp120) on phenotype and function of cultured monocytes. Rec.gp120 significantly reduced the accessory function of monocytes to stimulate autologous lymphocytes with anti‐CD3, the Fc receptor–mediated chemiluminescence of monocytes, and the expression of CD4 and Fc receptor I/II, while the expression of the monocyte marker GD14 and major histocompatibility complex class I and II was not influenced. According to these phenotypic results, preincubation of monocytes with rec.gp120 depressed anti‐CD3 antibody‐induced T cell stimulation and Fc receptor‐mediated phagocytosis as determined by chemiluminescence. Interferon‐γ release of lymphocytes induced by purified protein derivative of tuberculin was enhanced by gp120. These effects of isolated gp120 on monocyte immune functions in vitro might contribute to the understanding of the pathophysiology of HIV‐1 infection in viva J . Leukoc. Biol . 55: 545–551; 1994.