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Expression of p47‐ phox and p67‐ phox proteins in murine bone marrow–derived macrophages: Enhancement by lipopolysaccharide and tumor necrosis factor α but not colony stimulating factor 1
Author(s) -
Green Simon P.,
Hamilton John A.,
Uhlinger David J.,
Phillips Wayne A.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.4.530
Subject(s) - lipopolysaccharide , tumor necrosis factor alpha , biology , respiratory burst , nadph oxidase , macrophage , bone marrow , microbiology and biotechnology , western blot , priming (agriculture) , immunology , biochemistry , in vitro , enzyme , gene , botany , germination
We have investigated the relationship between the expression of the p47‐ phox and p67‐ phox cytosolic components of the NADPH oxidase and priming of the macrophage respiratory burst. Western blot analysis revealed that murine bone marrow‐derived macrophages (BMM) contain immunoreactive proteins detected by antisera raised against recombinant human p47‐ phox and p67‐phox. Priming BMM by exposure to tumor necrosis factor or (TNF‐ α ) or lipopolysaccharide (LPS) increased the levels of p47‐ phox and p67‐ phox. Colony‐stimulating factor 1 (CSF‐1), which we previously found to have a negative effect on the priming of murine macrophages, had no effect on the level of p47‐ phox but down‐regulated that of p67‐ phox. Our results suggest that the regulatory effects of LPS, TNF‐ α , and CSF‐1 on the respiratory burst of BMM may be due to modulation of the expression of the p47‐ phox and p67‐ phox cytosolic components of the NADPH oxidase. J. Leukoc. Biol. 55: 530–535; 1994.