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Fluoride prevents degranulation of the azurophilic and specific granules in electropermeabilized neutrophils
Author(s) -
Niessen Hans W.M.,
Kessels G.C. Roger,
Verhoeven Arthur J.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.4.489
Subject(s) - degranulation , dephosphorylation , gtp' , phosphatase , azurophilic granule , biology , microbiology and biotechnology , biochemistry , phosphorylation , immunology , enzyme , myeloperoxidase , receptor , inflammation
The effect of A1F 4 ‐ on the degranulation process in human neutrophils was investigated. In intact neutrophils, A1F 4 ‐ induced degranulation, whereas in electropermeabilized neutrophils A1F 4 ‐ did not stimulate degranulation. In electropermeabilized neutrophils, fluoride ions proved to be inhibitory for the degranulation induced by addition of Ca 2+ and/or GTP‐γ‐S. Another phosphatase inhibitor, okadaic acid, inhibited degranulation induced by Ca 2+ or by GTP‐γ‐S but not degranulation induced by the combination of Ca 2+ plus GTP‐γ‐S. It is concluded that under suboptimal conditions of stimulation with Ca 2+ or GTP‐γ‐S, protein dephosphorylation plays an important role in the degranulation response in human neutrophils. J. Leukoc. Biol. 55: 489–495; 1994.