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Slow calcium waves imaged in myeloid cells derived from neonatal cord blood
Author(s) -
Roberts G. M.,
Davies E. V.,
Hallett M. B.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.4.461
Subject(s) - biology , myeloid , cord blood , cytosol , myeloid cells , microbiology and biotechnology , population , cell , receptor , calcium , macrophage , immunology , medicine , biochemistry , in vitro , enzyme , environmental health
Myeloid cells were derived from neonatal cord blood by culture with granulocyte‐macrophage colony‐stimulating factor for approximately 8 days. The resultant cell population contained large adherent cells (diameter ≥90 μ m), expressing formylated peptide receptors that were functionally coupled to cytosolic free Ca 2+ signaling. Imaging of the cytosolic free Ca 2+ changes in these cells revealed initial focal release of Ca 2+ from a site from within the cell, with elevated Ca 2+ also near the cell edge. Increased cytosolic free Ca 2+ moved as a slow oscillating wave across the cell (velocity 1 μ m/s). As similar events may occur in mature neutrophils and monocytes but be difficult to resolve because of the small size of these cells, it was concluded that neonatal myeloid cells may provide a useful model system for the investigation of Ca 2+ signaling in myeloid cells. J. Lcukoc. Biol . 55: 461–466; 1994.