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Interleukin‐10 is a potent inhibitor of tumor cytotoxicity by human monocytes and alveolar macrophages
Author(s) -
Nabioullin Roustem,
Sone Saburo,
Mizuno Kazuto,
Yano Seiji,
Nishioka Yasuhiko,
Haku Takashi,
Ogura Takeshi
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.4.437
Subject(s) - cytotoxicity , monocyte , bronchoalveolar lavage , lipopolysaccharide , biology , interleukin , in vivo , immunology , effector , in vitro , pharmacology , microbiology and biotechnology , cytokine , medicine , lung , biochemistry
The effects of purified human interleukin‐10 (IL‐10) on the expression of antitumor activity of human monocytes and alveolar macrophages (AMs) obtained by centrifugal elutriation and bronchoalveolar lavage, respectively, from the same healthy donors were examined. Monocytes and AMs were incubated for 16 h in medium with lipopolysaccharide (LPS) in the presence or absence of IL‐10 or IL‐4, and then their tumoricidal activity was assayed by measuring 125 I‐IUdR release from human melanoma (A375) cells. Addition of IL‐10 to cultures of monocytes or AMs with LPS resulted in dose‐dependent suppression of their cytotoxicity against A375 cells, the suppression of the activity of monocytes being the higher. IL‐10 also suppressed the synergistic effects of interferon‐γ and desmethyl muramyldipeptide in activation of monocytes. IL‐10 inhibited the early induction phase of monocyte activation but not the effector phase (monocyte‐mediated cytotoxicity). IL‐10 plus IL‐4 inhibited the antitumor activities of AMs and monocytes much more than either IL‐10 or IL‐4 alone. IL‐10 and IL‐4 at sub‐optimal concentrations also showed synergistic inhibitory effects. These findings suggest that IL‐10 may be important in vivo in down‐regulating the antitumor activities of monocytes and AMs in the lung by inhibiting their productions of antitumor effector molecules. J. Leukoc. Biol. 55: 437–442; 1994.