Premium
LGL‐1: a potential triggering molecule on murine NK cells
Author(s) -
Mason Llewellyn H.,
Yagita H.,
Ortaldo John R.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.3.362
Subject(s) - biology , antibody dependent cell mediated cytotoxicity , lymphokine activated killer cell , interleukin 12 , monoclonal antibody , natural killer cell , interleukin 21 , microbiology and biotechnology , antigen , cytotoxicity , epitope , major histocompatibility complex , lymphokine , antibody , cytotoxic t cell , immunology , in vitro , cd8 , biochemistry
Natural killer (NK) cells mediate non‐major histocompatibility complex‐restricted lysis of tumor cells, lymphokine‐activated killing (LAK), antibody‐dependent cellular cytotoxicity (ADCC), and reverse ADCC (RADCC). LGL‐1 + cells identify a major subset (50%) of murine NK cells. Here we demonstrate that monoclonal antibodies (mAbs) to LGL‐1 consistently induce interleukin‐2‐cultured, and Corynebacterium parvum (in vivo)‐activated NK cells to induce RADCC. LGL‐1 triggering of activated NK cells coincides with enhanced LGL‐1 expression. Testing of murine mAbs to epitopes of CD2 only appears to augment RADCC induced by mAb NK‐1.1 on fresh NK cells. Immunoprecipitation of the LGL‐1 antigen reveals a highly disulfide‐linked 40‐kDa homodimer subunit that is N ‐glycosylated. Therefore, LGL‐1 may be similar to other recently characterized NK‐associated antigens such as NK‐1.1, Ly‐49, and NKR‐PI. We conclude that although LGL‐1 is expressed on “resting” NK cells, enhanced surface expression following activation is usually required for it to act as a signaling molecule. J . Leukoc. Biol. 55: 362–370; 1994.