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β 2 ‐Adrenoceptor agonists regulate the IL‐4‐induced phenotypical changes and IgE‐dependent functions in normal human monocytes
Author(s) -
PaulEugène Nathalie,
Kolb Jean Pierre,
Damais Chantal,
Abadie Annie,
MenciaHuerta Jean Michel,
Braquet Pierre,
Bousquet Jean,
Dugas Bernard
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.3.313
Subject(s) - cd23 , immunoglobulin e , biology , endocrinology , nitric oxide , integrin alpha m , monocyte , medicine , immunology , pharmacology , immune system , antibody
The β 2 ‐adrenoceptor agonists salbutamol and fenoterol were tested for their regulatory effects on human monocyte phenotype and functions, either alone or in combination with interleukin‐4 (IL‐4). These drugs enhanced in a dose‐dependent manner the IL‐4‐induced membrane and mRNA expression of the low‐affinity receptor for immunoglobulin E (IgE) (CD23), as well as the release of its soluble form, sCD23. Salbutamol and fenoterol alone elicited expression of the monomorphic β 2 ‐chain (CD18) of the leukocyte functional antigen (LEA 1 ) family. This effect appeared to be restricted to CD11b (CR3) and CD11c (gp 150–95), because CD11a (LFA‐1α chain) was not modified, β 2 ‐Adrenoceptor stimulation was also found to potentiate the effect of IL‐4 on CD11b, CD11c, and CD18 expression. In contrast, these agents alone did not alter the level of major histocompatibility complex class II and CD14 antigens or modify their respective up‐ and down‐regulation by IL‐4. Ligation of CD23 on IL‐4‐preincubated (CD23 * ) monocytes with IgE/anti‐IgE immune complexes induced the release of free radicals nitric oxide and of the proinflammatory mediators IL‐6 and thromboxane B 2 (TxB 2 ). Addition of salbutamol, inactive alone, potentiated the generation of superoxide anion and of nitric oxide generation, as well as the production of IL‐6 and TxB 2 triggered by CD23 ligation. These results indicate that β 2 ‐adrenoceptor stimulation potentiates in vitro the IL‐4‐induced phenotypical and functional changes on monocytes and suggest that such an interaction could occur in IgE‐dependent immune reactions. J. Leukoc. Biol. 55: 313–320; 1994.