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Determination of leukotriene effects on human neutrophil chemotaxis in vitro by differential assessment of cell motility and polarity
Author(s) -
Krauss A.H.P.,
Nieves A.L.,
Spada C.S.,
Woodward D. F.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.2.201
Subject(s) - chemotaxis , motility , leukotriene b4 , biology , chemotaxis assay , microbiology and biotechnology , cell , leukotriene , cell migration , in vitro , immunology , inflammation , biochemistry , receptor , asthma
The effects of leukotriene (LT) B 4 and D 4 on the motility of human peripheral blood neutrophils were investigated employing a novel analytical method. Using the under‐agarose technique, migration distance and vectorial orientation of neutrophils in response to selected LT concentrations were determined with the aid of digital image processing. Neutrophil polarization induced by a chemotactic gradient was very apparent even at fields taken adjacent to the cell seeding well where little directional cell motility had occurred. Thus, cell polarization appeared to be the earliest response to chemoattractive LTs. Cell motility occurred in a dose‐dependent manner to LTB 4 according to determination of the leading edge. LTD 4 produced similar effects on neutrophil polarization and motility, but these occurred only at very high concentrations. These data support the view that vectorial orientation is a prerequisite for directional migration of cells and it is also feasible that these are separately regulated events. Furthermore, our studies confirm that LTB 4 and, to a much lesser extent, LTD 4 are chemotactic for human peripheral blood neutrophils. J. Leukoc. Biol. 55: 201–208; 1994.