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Comparison of leukotriene B 4 and D 4 effects on human eosinophil and neutrophil motility in vitro
Author(s) -
Spada C. S.,
Nieves A.L.,
Krauss A.HP.,
Woodward D.F.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.2.183
Subject(s) - chemokinesis , leukotriene b4 , chemotaxis , eosinophil , leukotriene , leukotriene d4 , biology , motility , immunology , neutrophile , granulocyte , chemotaxis assay , leukotriene c4 , inflammation , microbiology and biotechnology , receptor , biochemistry , asthma
The motility of isolated normal human peripheral blood eosinophils and neutrophils in response to exogenous leukotrienes B 4 and D 4 was examined by means of a modified under‐agarose technique and a novel quantitative sampling strategy. Leukotriene D 4 was a potent chemoattractant for eosinophils, with a significant threshold chemotactic effect evident at 10 ‐10 M. The abolition of eosinophil chemotaxis by the potent and selective peptide‐leukotriene‐antagonist SK&F 104353 indicated the pharmacological specificity of the leukotriene D 4 ‐induced response. The chemokinetic response of eosinophils to leukotriene D 4 generally did not differ significantly from spontaneous migratory activity of unstimulated cells. Leukotriene D 4 did not, however, alter directed neutrophil motility until a very high concentration (10 ‐5 M) was achieved, although significant neutrophil chemokinesis relative to unstimulated movement was observed over the tested concentration range. Directional emigration of both eosinophils and neutrophils was induced by leukotriene B 4 at concentrations as low as 10 ‐8 M. Analysis of leukocyte orientations provided evidence that chemokinetic responses were not being interpreted as indications of chemotactic behavior. These studies suggest that leukotriene D 4 may behave as a potent and selective chemoattractant for human eosinophils at physiologically relevant concentrations. J . Leukoc. Biol. 55: 183–191; 1994.