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Distinct patterns of differentiation induced in the monocytic cell line Mono Mac 6
Author(s) -
ZieglerHeitbrock H.W. Löms,
Schraut Winfried,
Wendelgaß Petra,
Ströbel Marion,
Sternsdorf Thomas,
Weber Christian,
Aepfelbacher Martin,
Ehlers Monika,
Schütt Christine,
Haas Jürgen G.
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.1.73
Subject(s) - cd14 , biology , microbiology and biotechnology , cd23 , cell culture , monocyte , lipopolysaccharide , antibody , immunology , flow cytometry , immunoglobulin e , genetics
The human Mono Mac 6 cell line exhibits many characteristics of mature blood monocytes including expression of the CD14 molecule and production of cytokines, such as interleukin‐1 (IL‐1), IL‐6, and tumor necrosis factor. To determine whether these cells can be further differentiated, we treated the cells for up to 3 days with either prostaglandin E 2 (PGE 2 ; 10 ‐5 or 10 ‐6 M), lipopolysaccharide (LPS; 10–20 ng/ml), or tetradecanoylbhorbol‐13‐acetate (TPA; 10–50 ng/ml). All three re‐agents reduced proliferation and expression of the early myelomonocytic antigen CD33, and all increased phagocytosis of staphylococci and constitutive expression of mRNA for the macrophage colony‐stimulating factor (M‐CSF) receptor. By contrast, with respect to CD23 FcRII) expression, GD14 expression, and production of O 2 ‐ , the three reagents induced distinct responses. Expression of CD23 (FcRII) on Mono Mac 6 cells (36%) was not increased by LPS and TPA but was increased by PGE 2 treatment to 48%, with a 50% increase of fluorescence intensity. The CD14 antibody My4 stained more than 75% of untreated Mono Mac 6 cells with a specific mean fluorescence intensity of 87.5 channels. This staining was increased more than twofold by both PGE 2 and LPS. Staining with the GD14 antibody UGHM1 (6%) was increased to 43% by PGE 2 and to 43% by LPS. This increase in CD14 cell surface expression was accompanied by a rise in soluble CD14 and enhancement of CD14 mRNA. By contrast, TPA treatment resulted in a twofold decrease of CD14 cell surface staining with no significant change in sGD14, while CD14 mRNA was transiently down‐regulated. Secretion of O 2 ‐ (stimulated by TPA) was already detectable in untreated Mono Mac 6 cells 6.1 mmol/10 6 cells/30 min), and this response was enhanced 10‐fold by pretreatment with LPS but not with PGE 2 or TPA. The kinetics of M‐GSF receptor mRNA, CD14 expression, and O 2 ‐ production revealed that these monocytic features started to increase at 6–24 h and were maximal at 2 days. These data suggest that the three re‐agents induce maturation of the Mono Mac 6 cells to different levels or into different branches of the monocyte system with the notable differences that PGE 2 enhances CD23 expression, LPS enhances O 2 ‐ secretion, and TPA down‐regulates CD14. J. Leukoc. Biol . 55: 73–80; 1994.