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Ligation of N ‐acetylgalactosamine–containing structures on rat bone marrow cells enhances myeloid differentiation and murine granulocyte‐macrophage colony‐stimulating factor‐induced proliferation
Author(s) -
Krugluger Walter,
Köller Marcus,
Allmaier Martina,
BoltzNitulescu George,
Förster Othmar
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.55.1.127
Subject(s) - biology , bone marrow , progenitor cell , microbiology and biotechnology , myeloid , granulocyte macrophage colony stimulating factor , granulocyte , cellular differentiation , colony stimulating factor , in vitro , immunology , biochemistry , stem cell , gene , haematopoiesis
Previously we have reported the differentiation‐dependent expression of a soybean agglutinin (SBA)‐binding structure on rat bone marrow cells (BMCs) during their differentiation into macrophages (m ø s). In the present study we tried to analyze the functional role of the SBA‐binding structure in BMC proliferation and differentiation. Addition of SBA to BMC cultures driven into m ø differentiation by recombinant murine granulocyte‐macrophage colony‐stimulating factor (rmGM‐CSF), resulted in a two‐ to threefold increased proliferation rate compared with rmGM‐CSF alone. However, the number of colonies in methyl cellulose was not increased by SBA. The effect of SBA was dose dependent (from 4 to 83 pM SBA), with a maximum effect at 83 pM. Experiments to detect a possible synergistic effect of additional cytokines produced by BMC after SBA treatment were inconclusive. The enhancing effect of SBA was also seen when high‐density cells, which did not proliferate in response to rmGM‐CSF (mainly granulocytes), were removed. Therefore, SBA may increase the CSF reactivity of re‐sponsive m ø progenitor cells directly by binding to N ‐acetylgalactosamine residues on their surface. J. Leukoc. Biol. 55: 127–132; 1994.