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Both mannose and β‐glucan receptors are involved in phagocytosis of unopsonized, heat‐killed Saccharomyces cerevisiae by murine macrophages
Author(s) -
Giaimis Jean,
Lombard Wes,
Fonteneau Paul,
Muller Christian D.,
Levy Rachel,
MakayaKumba Madeleine,
Lazdins Janis,
Poindron Philippe
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.54.6.564
Subject(s) - laminarin , phagocytosis , biology , mannose receptor , mannose , microbiology and biotechnology , mannan , receptor , macrophage , antibody opsonization , saccharomyces cerevisiae , internalization , biochemistry , yeast , in vitro , opsonin , polysaccharide
We studied the involvement of lectin‐like receptors in phagocytosis of unopsonized heat‐killed yeast (Saccharomyces cerevisiae ) by murine macrophagelike cell lines and murine peritoneal resident macrophages. For this purpose we used a technique that allowed us to discriminate ingested and adsorbed heat‐killed yeast. The internalization can be partly inhibited by soluble polyosides such as laminarin (β‐glucan) or a‐ mannan. However, when they were used together (0.4 mg/ml α‐mannan and 0.4 mg/ml laminarin), almost complete inhibition of phagocytosis was obtained. These observations suggest that phagocytosis of unopsonized heat‐ killed yeast by murine macrophage‐like cell lines as well as murine peritoneal resident macrophages is mediated by both mannose and β‐glucan receptors. The respective activity of these two types of receptors is a function of in vitro cell differentiation. To achieve maximal phagocytosis of unopsonized heat‐killed yeast, coexpression of both mannose and β‐glucan receptors is required.