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Transforming growth factor β1 regulation of macrophage activation depends on the triggering stimulus
Author(s) -
Corradin Sally Betz,
BuchmullerRouiller Yolande,
Smith Josiane,
Suardet Laurent,
Mau§l Jacques
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.54.5.423
Subject(s) - transforming growth factor , lipopolysaccharide , macrophage , tumor necrosis factor alpha , biology , secretion , macrophage activating factor , prostaglandin e2 , cytokine , in vitro , medicine , microbiology and biotechnology , endocrinology , immunology , biochemistry
We have examined the effects of transforming growth factor β1 (TGF‐β1) on the regulation of murine bone marrow‐derived macrophage function. TGF‐β, added simultaneously with or up to 4 h before interferon‐γ (IFN‐γ) plus lipopolysaccharide (LPS), inhibited macrophage leishmanicidal activity, nitrite (NO 2 ”) production, and secretion of prostaglandin E 2 . In contrast, no effect of TGF‐β could be demonstrated on macrophages stimulated with IFN‐γ plus tumor necrosis factor‐γ (TNF‐γ) under the same conditions. These results suggested that TGF‐β inhibited LPS‐induced triggering of macrophage activation, which was confirmed by studies with IFN‐ γ‐primed cells. Interestingly, when macrophages were pretreated with TGF‐β for 24 h, NO 2 − production in response to IFN‐γ plus TNF‐α was also inhibited. Although control and IFN‐γ/LPS‐stimulated macrophages were found to secrete latent TGF‐β, only the IFN‐ γ/LPS cultures produced biologically active TGF‐β. Significantly, active TGF‐β was present at concentrations shown earlier to inhibit macrophage function.