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Analysis of interleukin‐2‐activated killer cells of rhesus monkeys: striking resemblance to the human system
Author(s) -
Savary Cherylyn A.,
Lotzová Eva,
Jackson Helen J.,
Jardine John H.,
Ang K. Kian
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.54.4.307
Subject(s) - biology , cd16 , cd8 , immunology , cd38 , spleen , interleukin 2 , bone marrow , interleukin , oncolytic virus , il 2 receptor , microbiology and biotechnology , cd3 , immune system , t cell , cytokine , cd34 , stem cell
We have found numerous and exquisite homologies between the interleukin‐ 2 (IL‐ 2 )‐activated killing systems of rhesus monkeys and humans. Lymphocytes with high oncolytic and proliferative activity were generated from peripheral blood, spleen, and bone marrow of monkeys sifter culture with IL‐2. The distribution of lymphocyte subsets in IL‐2 cultures closely paralleled that seen in humans, including a decrease in GD4 + and increase in CD8 + , CD38 + , and CD25 + lymphocytes and an increase in density of GD2 molecules. We also describe three distinct subsets of monkey lymphocytes, GD16 + ,56 − , CD16 + 56 +dim ”, and CD16‐,56 bright , and show that the CD56 +bright ” subset is substantially increased (to as high as 79%) after IL‐2 activation. Furthermore, as in humans, the cells with oncolytic activity were characterized as CD56 + CD16 +‐ , and CD8 + . This strong homology with humans indicates that the rhesus monkey may be a valuable preclinical model for evaluation of therapeutically relevant biological response modifiers.