Premium
Suppression of human IgE antibody forming cell responses by IL‐6
Author(s) -
Kleiner Gary I.,
Auci Dominick L.,
Shaikh Altaf,
Chice Soto M.,
Durkin Helen G.
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.54.2.168
Subject(s) - immunoglobulin e , elispot , biology , immunology , antibody , cytokine , interleukin 4 , immune system , t cell
To study the effects of cytokines on human IgE antibody forming cells (AFCs), log phase U266 myeloma cells (3 × 10 3 /ml), which secrete immunoglobulin E (IgE), were cultured for 0‐24 h with and without cytokine or with or without antibodies against various cytokines. The numbers of IgE AFCs were determined in ELISPOT assay. We found that interleukin‐6 (IL‐6) suppressed (to 95%) whereas anti‐IL‐6 increased (to 148%) the numbers of IgE AFCs and that both worked in a dose‐ dependent fashion. IL‐4 and interferon‐γ (IFN‐γ) also suppressed IgE AFC responses in a dose‐dependent fashion. However, antibodies to these cytokines had no effect. In contrast, IFN‐α increased (to fourfold) the numbers of IgE AFCs in a dose‐dependent fashion. The data are the first to show a suppressive effect of IL‐6 on human IgE responses and may also suggest a role for IL‐6 in the treatment of atopic disease.