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The leukocyte surface antigens CD11b and CD18 mediate the oxidative burst activation of human peritoneal macrophages induced by type 1 fimbriated Escherichia coli
Author(s) -
Gbarah Awni,
Gahmberg Carl G.,
Boner Geoffrey,
Sharon Nathan
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.54.2.111
Subject(s) - biology , cd18 , integrin alpha m , respiratory burst , microbiology and biotechnology , monoclonal antibody , antigen , antibody , biochemistry , immunology , flow cytometry
Abstract Analysis by immunofluorescence‐activated cell sorting of human peritoneal macrophages from patients undergoing intermittent peritoneal dialysis revealed that they express the CD11/CD18 surface antigens, with CDllb and CD18 as the predominant ones. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of im‐ munoprecipitates obtained from lysates of 125 I‐labeled macrophages with rabbit polyclonal antibodies against the CDlla‐c/CD18 complex or against CD18, revealed four radioactive bands corresponding to CDlla, CDllb, CDllc, and CD18. Monoclonal antibodies against CDllb and CD18 inhibited by 80 and 90%, respectively, the oxidative burst activation of the macrophages by type 1 fimbriated Escherichia coli, whereas monoclonal antibodies against CDlla, CDllc, and CD43 were without effect. Our results suggest that CDllb and CD18 (receptors for C3bi) serve also as receptors for mannose‐specific E. coli on human peritoneal macrophages and may be involved in the lectinophagocytosis of the bacteria by these cells.