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Comparison of cytosolic calcium shifts, superoxide generation, and lactoferrin secretion in the neutrophils of atopies and nonatopics
Author(s) -
Zweiman Burton,
Allmen Carolyn,
Moskovitz Anne,
Atkins Paul,
Taylor Megan,
Korchak Helen
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.6.727
Subject(s) - lactoferrin , secretion , superoxide , exocytosis , biology , cytosol , endocrinology , immunology , leukotriene b4 , medicine , microbiology and biotechnology , biochemistry , inflammation , enzyme
We previously found that platelet‐activating factor (PAF) stimulated greater lactoferrin secretion by neutrophils of atopies than nonatopics. To help understand underlying mechanisms, we compared agonist‐ stimulated lactoferrin secretion with another response, superoxide generation, to determine whether there is a global alteration in the reactivity of atopic neutrophils to these stimuli. We also determined Ca 2+ mobilization in such neutrophils because such mobilization is a signaling pathway for both superoxide generation and granule content exocytosis. Although PAF again stimulated greater lactoferrin secretion in atopic than in nonatopic neutrophils, the peak superoxide secretion and cytosolic Ca 2+ mobilization were not significantly different in atopies and nonatopics. Leukotriene B 4 ‐induced superoxide secretion and cytosolic Ca 2+ shifts were also similar in atopies and nonatopics. These findings suggest that (1) the enhanced PAF‐induced lactoferrin secretion in atopic neutrophils is not a reflection of greater PAF binding or broad‐based cell hyperreactivity and (2) a selective signaling pathway in atopic neutrophils may be responsible for the enhanced lactoferrin secretion. These findings may be relevant to in vivo events because we have found increased PAF and lactoferrin release in skin chambers overlying immunoglobulin E‐mediated human skin reactions.