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Effects of macrophage‐colony stimulating factor on human monocytes: Induction of expression of urokinase‐type plasminogen activator, but not of secreted prostaglandin E 2 , interleukin‐6, interleukin‐1, or tumor necrosis factor‐a
Author(s) -
Hamilton John A.,
Whitty Genevieve A.,
Stanton Heather,
Meager Anthony
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.6.707
Subject(s) - biology , granulocyte macrophage colony stimulating factor , plasminogen activator , tumor necrosis factor alpha , monocyte , prostaglandin e2 , interleukin , endocrinology , macrophage , cytokine , macrophage colony stimulating factor , lipopolysaccharide , medicine , immunology , microbiology and biotechnology , in vitro , biochemistry
It is often assumed that macrophage‐colony stimulating factor (M‐CSF) or CSF‐1, as well as granulocyte macrophage‐CSF (GM‐CSF), can induce inflammatory mediator production by monocytes/macrophages. We demonstrate with elutriation‐purified human monocytes that, in contrast to lipopolysaccharide, recombinant human CSF‐1 does not induce secretion of prostaglandin E 2 , interleukin‐6 (IL‐6), IL‐lβ, or tumor necrosis factor a, as measured by immunoassay; however, increased urokinase‐type plasminogen activator (u‐PA) activity in cell lysates and mRNA was observed. Similar results were obtained when the monocytes were treated with recombinant human GM‐CSF. Such increased u‐PA expression may contribute to the function of CSF‐1 at sites of inflammation.