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Analysis of differential HLA‐DQB expression in autologous B cell lines
Author(s) -
Woolfrey Ann E.,
Andersen L. Christina,
Shewey Linda,
Chung JoSan,
Nepom Gerald T.
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.6.697
Subject(s) - biology , gene , human leukocyte antigen , transfection , microbiology and biotechnology , cell culture , gene expression , regulation of gene expression , regulatory sequence , genetics , antigen
Glass II major histocompatibility complex genes are differentially expressed during cellular activation and differentiation, often in a locus‐specific manner. We investigated the differential expression of the HLA‐ DQB gene, using B cell lines LAZ221 and LAZ388: LAZ221, derived from an early B cell leukemia, expresses HLA‐DR but not HLA‐DQ LAZ388, the autologous Epstein‐Barr virus‐transformed B cell line, expresses both DR and DQ Transfection experiments demonstrate differential function of class II gene upstream regulatory regions in the two lines, which correlates with differential class II gene expression. Using gel retardation and DNase I footprint assays, we demonstrate that absence of DQB gene expression is associated with characteristic nuclear protein‐binding interactions in the proximal DQB gene upstream regulatory region. These interactions are visualized as DNA‐protein complexes that are seen with nuclear proteins from the DQ‐negative cell line, LAZ221, and involve consensus promoter Y box and W box elements, as well as novel upstream sites. Transcriptional regulatory proteins that differ in these autologous B cell lines may be stage‐specific factors involved in the developmental regulation of HLA genes.