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Recombinant bovine GM‐CSF primes superoxide production but not degranulation induced by recombinant bovine interleukin‐1 β in bovine neutrophils
Author(s) -
Tao Weng,
Dougherty; Ruth,
Johnston Paul,
Pickett Walter
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.6.679
Subject(s) - degranulation , recombinant dna , biology , superoxide , immunology , microbiology and biotechnology , biochemistry , enzyme , gene , receptor
Bovine neutrophil activation, superoxide production, and β‐glucosaminadase release induced by various biological stimuli were examined. Plateletactivating factor (PAF) and recombinant bovine interleukin‐1 β (r‐BoIL‐1β) induced superoxide production and β ‐glucosaminadase release in bovine neutrophils. When these two responses were compared, the dose requirement for maximum activation was similar for PAF (1 × 10 −6 M). However, the concentration of r‐BoIL‐Ι β required for the maximum degranulation (2.5 × 10 −7 M) was 100‐fold higher than that for the maximum superoxide production (2.5 × 10‐ 9 M). Furthermore, pretreatment of cells with recombinant bovine granulocyte‐ macrophage colony‐stimulating factor (r‐BoGM‐CSF) enhanced both superoxide production and β ‐glucosaminidase release induced by PAF. In contrast, whereas superoxide production induced by r‐BoIL‐1 β was enhanced by r‐ BoGM‐CSF priming, β ‐glucosaminidase release induced by r‐BoIL‐l β was significantly reduced by pretreatment with r‐BoGM‐CSF. CL 184,005, a PAF antagonist, inhibited PAF‐induced glucosaminidase release and superoxide production but did not inhibit r‐BoIL‐l β ‐in‐ duced superoxide production and degranulation. In addition, it did not inhibit the priming effect of r‐BoGM‐ GSF on r‐BoIL‐l β ‐induced superoxide production. These results suggest that (1) PAF and r‐BoIL‐1 β activate bovine neutrophils by different mechanisms, (2) r‐BoGM‐CSF primes superoxide production and degranulation induced by PAF, (3) r‐BoGM‐CSF primes superoxide production but not degranulation induced by r‐BoIL‐l β , and (4) the priming effect of r‐BoGM‐CSF is not mediated by PAF.