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Multiple pathways of interferon‐induced gene expression in murine macrophages
Author(s) -
Politis Alexander D.,
Sivo Judit,
Vogel Stefanie N.
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.5.583
Subject(s) - biology , protein kinase c , interferon , microbiology and biotechnology , protein kinase a , gene , messenger rna , signal transduction , gene expression , kinase , antigen , macrophage , immunology , genetics , in vitro
Abstract Steady‐state levels of mRNAs that encode specific FcyR and la antigen genes have been measured in macrophages treated with interferons (IFNs) to examine the induction of these markers at the molecular level. Our previous studies suggested requirement for protein kinase C (PKC) in the IFN induction of these macrophage surface markers, although a difference in PKC dependence was found between IFN‐α/ β ‐ and IFN‐γ‐induced FcγR expression. The protein kinase antagonist H 7, used previously to distinguish between the surface induction of FcyR by IFN‐α/β and IFN‐γ, also distinguishes between the IFN‐γ and IFN‐γ in the induction of FcyRI mRNA and FcyRI surface expression. Protein kinase inhibitors blocked the IFN‐γ induction of la mRNA in a manner similar to that reported previously for cell surface la expression. It is concluded that FcyRI is induced by both IFN‐γ and IFN‐γ through distinct biochemical pathways, whereas IFN‐γ utilizes distinct pathways to induce the two macrophage activation markers, la antigen and FcyRI.