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An immunohistologic study of granulomatous inflammation in SIV‐infected rhesus monkeys
Author(s) -
Horvath Christopher J.,
Hunt Ronald D.,
Simon Meredith A.,
Sehgal Prabhat K.,
Ringler Douglas J.
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.5.532
Subject(s) - biology , epithelioid cell , cd8 , giant cell , simian immunodeficiency virus , pathology , high endothelial venules , antigen , cd20 , cd3 , inflammation , cd68 , immunology , immunohistochemistry , virus , medicine , genetics
We studied granulomatous inflammation in simian AIDS using histologic, immunohistologic, and in situ hybridization techniques. Complete Freund's adjuvant was used to induce granulomas in two control animals and two macaques infected with simian immunodeficiency virus (SIV) and having low peripheral CD4 + T cell counts. Control animals developed large (>2 cm diameter) epithelioid granulomas containing CD68 + macrophages (mφs), epithelioid m φ s and multinucleated giant cells (MNGCs), CD4 + and CD8 + T cells, and small perivascular collections of CD20 + B cells. Lymphocytes rarely expressed proliferating cell nuclear antigen (Ki‐67), and only rare endothelial cells expressed vascular cell adhesion molecule 1 (VCAM‐1). In contrast, SIV + animals had smaller (<0.5 cm diameter) epithelioid granulomas characterized by numerous large, dense CD8 + , CD20 + lymphocyte aggregates with prominent local division (Ki‐67 + ). Despite low blood CD4 + T cell numbers, there was a substantial CD4 + T cell infiltrate, accompanied by enhanced endothelial VCAM‐1 expression. These granulomas contained no detectable SIV antigen or RNA. Thus, in simian AIDS, experimentally induced granulomatous responses are grossly attenuated, yet associated with increased local endothelial‐leukocyte signaling and lymphocyte division.