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Polyunsaturated fatty acids increase neutrophil adherence and integrin receptor expression
Author(s) -
Bates Edna J.,
Fèrrante Antonio,
Harvey Dianne R,
Poulos Alt
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.4.420
Subject(s) - polyunsaturated fatty acid , eicosapentaenoic acid , docosahexaenoic acid , biology , proinflammatory cytokine , arachidonic acid , fish oil , receptor , fatty acid , biochemistry , integrin alpha m , receptor expression , pharmacology , medicine , immunology , endocrinology , inflammation , enzyme , fishery , fish <actinopterygii>
Fish oils are abundant in polyunsaturated fatty acids of the n‐3 series (in particular eicosapen‐ taenoic, 20:5 and docosahexaenoic acid, 22:6). Such fatty acids are generally considered to be beneficial in the prevention of cardiac disease and to have anti‐inflammatory properties. Neutrophil adherence is an essential early event in an acute inflammatory response, and we have demonstrated that both 20:5 and 22:6 stimulate adherence in vitro. Arachidonic acid (20:4, n‐6) was also stimulatory. Significant stimulation of adherence was seen from 5 to 80 μ M (nontoxic concentrations) 22:6, 20:5, or 20:4. At the lower fatty acid concentrations tested (≤40 μ M) 20:5 was less active than 22:6 or 20:4 at stimulating adherence. Above 40 μ M there was no difference in the ability of the three fatty acids to stimulate adherence. At the lower fatty acid concentrations tested (≤10 μ M) 22:6 was less active than 20:4, whereas above 10 μ M they were equally active. Immunofluorescent flow cytometric analysis of neutrophil integrin (adherence) receptors showed that the complement G3bi receptor (GDI lb) was up‐regulated by these fatty acids. There was no change in GDI la or GDllc. Saturated fatty acids of the same chain length were without effect on adherence or receptor expression. The findings suggest that these polyunsaturated fatty acids may, under certain conditions, be proinflammatory with respect to their acute effects on the interaction of neutrophils with microbes, endothelium, and other tissues.