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Characterization of neutrophil activation by repeated injection of endotoxin in rabbits. Role of neutrophils in the generalized Shwartzman reaction
Author(s) -
Toh Hiroto,
Miyata Tadanori,
Torisu Motomichi
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.3.256
Subject(s) - integrin alpha m , in vivo , immunology , chemotaxis , cd18 , neutrophile , tumor necrosis factor alpha , biology , monoclonal antibody , in vitro , endothelial stem cell , inflammation , antibody , flow cytometry , receptor , biochemistry , microbiology and biotechnology
The relationship between activated neutrophils and end‐organ injury in endotoxemia was studied. The function of peripheral blood neutrophils (PMNs) in rabbits with the generalized Shwartzman reaction (GSR) was compared to that of PMNs rabbits receiving a single injection of endotoxin. The following results were obtained: (1) PMNs from rabbits with the GSR demonstrated enhanced adherence to endothelial cells and increased mitochondrial ATP production; (2) the GSR did not enhance chemotaxis and oxygen radical production of PMNs; (3) a single injection of endotoxin did not cause necrosis of visceral organs; (4) in vitro detachment of endothelial cells by PMNs was increased in rabbits with the GSR; (5) in vivo administration of monoclonal antibody (mAb) against CD1lb/CD 18 (Mac‐1) suppressed the increase in PMN adherence; and (6) hemorrhagic necrosis did not occur when mAb to Mac‐1 was injected. Thus, enhanced adherence of PMNs to endothelial cells appears to play a key role in endotoxin‐induced end‐organ injuries in this animal model.