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Vitamin D3 compounds regulate human immunodeficiency virus type 1 replication in U937 monoblastoid cells and in monocyte‐derived macrophages
Author(s) -
Pauza C. David,
Kornbluth. Richard,
Emau Peter,
Richman Douglas D.,
Deftos Leonard J.
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.2.157
Subject(s) - biology , monocyte , u937 cell , human immunodeficiency virus (hiv) , virology , vitamin , replication (statistics) , macrophage , viral replication , immunology , virus , cell culture , genetics , biochemistry , in vitro
Abstract We studied the effects of vitamin D3 compounds on the replication of human immunodeficiency virus type 1 (HIV‐1) in the monoblastoid cell line U937 and in primary monocyte‐derived macrophage cultures to understand how modulators of monocyte/macrophage effector function might affect the pathogenesis of HIV‐1 infection. U937 cell cultures exposed to l,a25‐dihydro‐ xyvitamin D3 prior to HIV‐1 infection showed enhanced virus replication that was apparently due to increased cellular resistance to viral cytopathic effects; a marked inhibition of virus replication was noted in cells exposed to 1 a, 25‐dihydroxyvitamin D3 subsequent to infection. Exposure of blood‐derived monocyte/macrophages to vitamin D3 compounds prior to infection also affected virus growth; in most cases, substantial inhibition of HIV‐ 1 replication was noted in vitamin D3‐treated macrophage cultures. Our results demonstrate that vitamin D3 compounds with recognized abilities to induce cellular differentiation can modulate HIV‐1 infection of human macrophages.