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Regulation of herpes simplex virus type 1 gene expression in nonpermissive murine resident peritoneal macrophages
Author(s) -
Wu Linxian,
Morahan Page S.,
Leary Kathryn
Publication year - 1993
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.53.1.61
Subject(s) - biology , herpes simplex virus , vero cell , gene expression , promoter , gene , transcription (linguistics) , virology , microbiology and biotechnology , regulation of gene expression , virus , genetics , linguistics , philosophy
Abstract The restriction of herpes simplex virus type 1 (HSV‐1) gene expression in nonpermissive murine resident peritoneal macrophages (ResPMO) was analyzed at the level of transcription. Using nuclear run‐on assays, we established that transcription of a representative gene from each HSV‐1 kinetic class was initiated in both ResPMO and permissive Vero cells. Using transient expression assays, we observed that the promoters of two early genes were activated by the immediate‐early protein ICP4 in ResPMO. However, the level of trans‐ activation of these promoters by another immediate‐early protein, ICPO, was markedly reduced in ResPMO. Furthermore, the synergistic trans‐activation normally observed between ICPs 4 and O in Vero cells was noticeably absent in ResPMO. The data indicate that the block in viral gene expression in ResPMO occurs after initiation of viral gene transcription and involves dysfunction of a viral immediate‐early regulatory protein, ICP0.