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Role of CD4 + T lymphocytes and interleukin‐5 in antigen‐induced eosinophil recruitment into the site of cutaneous late‐phase reaction in mice
Author(s) -
Iwamoto Itsuo,
Tomoe Sanae,
Tomioka Hisao,
Takatsu Kiyoshi,
Yoshida Sho
Publication year - 1992
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.52.5.572
Subject(s) - eosinophil , ovalbumin , eosinophilia , antigen , biology , cd8 , immunoglobulin e , interleukin 5 , immunology , infiltration (hvac) , monoclonal antibody , interleukin , microbiology and biotechnology , antibody , chemistry , cytokine , materials science , asthma , composite material
Previous studies suggested that the eosinophil recruitment into the site of cutaneous late‐phase reaction (LPR) was dependent on IgE antibody and mast cells. In this study, we determined the role of CD4 + T cells and CDS +, T cells in causing antigen‐induced eosinophil recruitment of LPR in mouse skin. Eosinophil infiltration into the subcutaneous tissue of ovalbumin (OVA)‐ sensitized BALB/c mice was biphasic, reaching the first peak at 6 h after the subcutaneous challenge with OVA and the second peak at 24 to 48 h. The in vivo depletion of CD4 + T cells by pretreatment with anti‐L3T4 monoclonal antibody (mAb) significantly decreased the second peak (at 24 h and 48 h), but not the first peak (at 6 h), of OVA‐induced eosinophil infiltration into the skin of OVA‐sensitized mice. However, the depletion of CD8 + T cells by pretreatment with anti‐Lyt‐2 mAb had no significant effect on either the first peak or second peak of OVA‐induced cutaneous eosinophilia. Pretreatment with anti‐murine interleukin‐5 (IL‐5) mAb also decreased the second peak, but not the first peak, of OVA‐ induced cutaneous eosinophilia. In contrast to the inhibitory effects of depletion of CD4+ T cells and of anti‐IL‐5 mAb on the second peak of antigen‐induced cutaneous eosinophilia, disodium cromoglycate and a selective antagonist for platelet activating factor (PAF) CV‐6209 decreased the first peak of OVA‐induced cutaneous eosinophilia in the mouse. These results indicate that CD4 + T cells, but not CD8 + T cells, cause the second peak of antigen‐induced eosinophil recruitment of cutaneous LPR and that IL‐5 mediates this eosinophil recruitment. In contrast, the first peak of antigen‐induced eosinophil recruitment of cutaneous LPR is mediated by mast cells and PAF.