Premium
RAPID COMMUNICATION: A synthetic peptide homologous to retroviral envelope protein down‐regulates TNF‐α and IFN‐γ mRNA expression
Author(s) -
Haraguchi Soichi,
Good Robert A.,
Cianciolo George J.,
Day Noorbibi K.
Publication year - 1992
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.52.4.469
Subject(s) - biology , messenger rna , peptide , microbiology and biotechnology , homologous chromosome , envelope (radar) , virology , genetics , gene , biochemistry , telecommunications , radar , computer science
We investigated the influence of CKS‐17, a synthetic heptadecapeptide that corresponds to a highly conserved domain of the immunosuppressive retroviral envelope protein pl5E, on staphylococcal enterotoxin B (SEB)‐induced TNF‐α gene expression in human peripheral blood mononuclear cells and highly purified human monocyte preparations, as well as the production of TNF‐ αprotein, using human peripheral blood mononuclear cells. RNA hybridization studies show that CKS‐17 inhibits SEB‐induced TNF‐α mRNA accumulation in human peripheral blood mononuclear cells and human monocytes. CKS‐17 is also shown to be highly suppressive for SEB‐induced production of TNF‐α proteins. Similarly, CKS‐17 inhibits expression of SEB‐induced IFN‐γ mRNA in human peripheral blood mononuclear cells. These results suggest that CKS‐17 down‐regulates both TNF‐α and IFN‐γ production at mRNA level.