C‐reactive protein selectively enhances the intracellular generation of reactive oxygen products by IgG‐stimulated monocytes and neutrophils

The acute phase protein, C‐reactive protein (CRP), when heat‐aggiegated (Agg‐CRP), potentiates immunoglobulin G (IgG) Fc receptor‐mediated luminol‐ enhanced chemiluminescence (CL) in human monocytes and neutrophils. Luminol‐CL is a sensitive measure of phagocyte respiratory burst activity; however, the nature of oxidative products contributing to the light emission md their site of generation remain incompletely defined. To more precisely describe the oxidative burst of monocytes and neutrophils to Agg‐CRP, superoxide anion release was measured by cytochrome c reduction. In addition, the extracellular release of hydrogen peroxide was distinguished from hydrogen peroxide generation using a phenol red oxidation assay. Finally, a flow cytometric de‐;ermination of dichlorofluorescin (DCFH) oxidation was employed as an index of intracellular peroxide production. Although Agg‐CRP alone did not stimulate hydrogen peroxide generation by either monocytes or neutrophils, it significantly enhanced hydrogen peroxide generation in response to heat‐aggregated IgG (Agg‐IgG). In contrast, Agg‐CRP did not enhance the extracellular release of either hydrogen peroxide or superoxide anion from Agg‐IgG‐stimulated cells. The capacity of Agg‐CRP to enhance selectively intracellular oxidative product generation was confirmed when measuring DCFH oxidation in Agg‐IgG‐stimulated cells. To evaluate whether this selective enhancement of intracellular oxidative events could be attributed, at least in part, to a scavenging effect of Agg‐CRP, a cell‐free oxygen radical‐generating system was employed. Agg‐CRP did not significantly liminish the lucigenin‐amplified CL response induced by the xanthine/xanthine oxidase reaction. These results indicate that although Agg‐CRP enhances the intracellular generation of reactive oxygen intermediates by monocytes md neutrophils, extracellular release of those products is lot influenced by cell interaction with Agg‐CRP. It is tempting to speculate that CRP can selectively boost the microbicidal activities of monocytes and neutrophils within an inflammatory site by amplifying the intracellular generation of reactive oxygen products without increasing damage to surrounding normal tissues.