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Low response of BALB/c macrophages to priming and activating signals
Author(s) -
Oswald Isabelle P.,
Afroun Samia,
Bray Dorothy,
Petit JeanFrançois,
Lemaire Genevieve
Publication year - 1992
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.52.3.315
Subject(s) - lipopolysaccharide , biology , mastocytoma , macrophage , priming (agriculture) , nitric oxide , microbiology and biotechnology , immunology , stimulation , balb/c , cytotoxicity , in vitro , immune system , biochemistry , endocrinology , botany , germination
Trehalose dimycolate (TDM), a mycobacterial glycolipid, is a powerful macrophage‐priming agent. However, its efficiency seems limited in the case of BALB/c mice. Peritoneal macrophages harvested from TDM‐treated BALB/c mice did not control BCG growth in vitro as efficiently as similar macrophages from two other mouse strains, (B6 × D2)F1 and C57BL/6, which are respectively Bcg r and Bcg s . BALB/c macrophages elicited by TDM also exhibited a low capacity to produce hydrogen peroxide and, after activation by lipopolysaccharide (LPS), weak cytostatic activity against P815 mastocytoma cells. Finally, alkaline phosphodiesterase, a marker of resident and inflammatory macrophages, was still expressed at a high level in macrophages of BALB/c mice treated with TDM. Low responsiveness of BALB/c macrophages to stimuli was not observed with TDM only; activation for tumor cytotoxicity of thioglycolateelicited macrophages from BALB/c mice required also higher doses of interferon‐γ, and LPS. l‐Arginine–dependent production of nitric oxide was inducible in macrophages from BALB/c mice, but the conditions required for its induction were more stringent. Thus, the reduced antiproliferative effects of BALB/c macrophages may be due to uncomplete induction of NO synthase after suboptimal stimulation.