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Lytic effects of Actinobacillus actinomycetemcomitans leukotoxin on human neutrophil cytoplasts
Author(s) -
Iwase Masayasu,
Korchak Helen M.,
Lally Edward T.,
Berthold Peter,
Taichman Norton S.
Publication year - 1992
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.52.2.224
Subject(s) - cytoplast , actinobacillus , biology , neutrophil extracellular traps , lytic cycle , extracellular , microbiology and biotechnology , lysis , exocytosis , immunology , cytoplasm , membrane , biochemistry , bacteria , inflammation , virus , genetics
Actinobacillus actinomycetemcomitans leukotoxin lysed human neutrophil cytoplasts. The reaction was associated with a rapid influx of extracellular calcium, a collapse in membrane potential, release of lactate dehydrogenase, and overt disintegration of the plasma membrane. These functional and structural alterations in the plasmalemma of neutrophil cytoplasts reinforce the hypothesis that A. actinomycetemcomitans leukotoxin acts as a pore‐forming, membranolytic agent and indicate that neutrophil cytoplasts are useful tools in studying the biology of membrane‐active toxins.