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Assessment of neutrophil leukocyte secretory response to fMLP in whole blood in vitro
Author(s) -
Chadwick Vinton S.,
Ferry Dianne M.,
Butt Terence J.
Publication year - 1992
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.52.2.143
Subject(s) - whole blood , biology , chemotaxis , in vitro , granulocyte , endocrinology , neutrophile , bioassay , medicine , immunology , n formylmethionine leucyl phenylalanine , pharmacology , biochemistry , genetics , receptor
A simple, precise method has been developed for assessing neutrophil secretory responses (release of vitamin B 12 binding protein from specific granules) to challenge of aliquots of whole blood with the bacterial chemotactic peptide N ‐formylmethionyl‐leucyl‐phenylalanine (fMLP). Dose‐response studies performed on blood from normal healthy volunteers showed higher maximal secretory responses in males than females (33.3 ± SEM 2.2 vs. 27.4 ± 2.5, P <.005) a left shift in dose‐response curves after feeding compared to fasting ( P <.005), spontaneous up‐regulation of responses in blood incubated at 37° for 1 h, and marked up‐regulation in response to preincubation with endotoxin. This whole blood challenge method may be used to study neutrophil responses in groups of individuals or patients without the confounding effects of changes in cell responses resulting from cell isolation procedures. The method may also be used as a bioassay for neutrophil‐activating factors.