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Synergism of interleukin 6 and 1α,25‐dihydroxyvitamin D 3 in induction of myeloid differentiation of human leukemic cell lines
Author(s) -
Duits Ashley J.,
Dimjati Wati,
Winkel Jan G.J.,
Capel Peter J.A.
Publication year - 1992
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.51.3.237
Subject(s) - biology , u937 cell , myeloid , lipopolysaccharide , integrin alpha m , cellular differentiation , cd14 , monocyte , microbiology and biotechnology , cell culture , immunology , biochemistry , flow cytometry , genetics , gene
Interleukin 6 (IL‐6) is a multifunctional cytokine with an important role in immunity. We analyzed the effect of recombinant human IL‐6 in combination with 1α,25‐dihydroxyvitamin D 3 (Vit. D 3 ) on differentiation of the human myeloid leukemic cell lines U937 and HL‐60 with respect to alterations in antigen expression and functional activity. Of a panel of antigens analyzed, only CD11b (the α chain of CR3), and GD14 (a cell surface protein recognizing the lipopolysaccharide‐binding protein‐lipopolysaccharide complex) had significantly increased expression. Expression of ICAM‐1 (CD54), a ligand for LFA‐1, was also found to be enhanced with a concomitant increase of ICAM‐1 mRNA levels. Enhanced nonspecific esterase levels and induction of respiratory burst activity confirmed that cell differentiation was induced. Furthermore, IL‐6 and Vit. D 3 had a profound effect on functional activities, as shown by enhancement of rosetting between sheep erythrocytes, sensitized with C3bi (EAC), and either U937 or HL‐60 cells. Also, phorbol myristate acetate–induced homotypic adhesion of U937, which is ICAM‐1 dependent, was markedly induced by these agents. These results indicate an important role of IL‐6 and Vit. D 3 in myeloid cell Function and development.