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Cryopreserved Cytoplasts From Human Polymorphonuclear Leukocytes (Cytokineplasts) Are Chemotactic at Speeds Comparable to Those of Fresh Intact Cells
Author(s) -
Malawista Stephen E.,
BoisfleuryChevance Anne
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.50.3.313
Subject(s) - biology , cryopreservation , chemotaxis , cytoplast , immunology , microbiology and biotechnology , andrology , cytoplasm , embryo , biochemistry , receptor , medicine
Abstract Cytokineplasts (CKP) are granule‐poor cytoplasts from human polymorphonuclear leukocytes (PMN) that retain motile function, even (unlike the parent PMN) after cryopreservation. Employing time‐lapse videomicroscopy, we examined the chemotactic properties of CKP after cryopreservation toward erythrocytes lysed by laser microirradiation. Paths of locomotion were plotted for six CKP in the field, and velocities were calculated at 10‐sec intervals. Mean velocities of the six fragments, ranging from 9.3 to 20.8 μm/min, are of the order of fresh, intact PMN, the fastest of locomoting cells.