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Role of 5‐Lipoxygenase Products of Arachidonic Acid in Cell‐to‐Cell Interaction Between Macrophages and Natural Killer Cells in Rat Spleen
Author(s) -
Chang KeJian,
Saito Hiroyuki,
Tatsuno Ichiro,
Tamura Yasushi,
Yoshida Sho
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.50.3.273
Subject(s) - arachidonic acid , leukotriene b4 , biology , macrophage , arachidonate 5 lipoxygenase , natural killer cell , leukotriene , splenocyte , eicosapentaenoic acid , lipoxygenase , spleen , phagocytic cell , ingestion , monocyte , cell , cytotoxic t cell , biochemistry , inflammation , immunology , phagocytosis , polyunsaturated fatty acid , enzyme , fatty acid , in vitro , asthma
In the present study we performed experiments to reveal the role off 5‐lipoxygenase products of arachidonic acid in cell‐to‐cell interaction between rat peritoneal macrophages and spleen natural killer (NK) cells. Peritoneal macrophages were found to significantly enhance NK cell activity. It was found that the enhancing activity of macrophages was significantly inhibited by the ingestion of highly purified eicosapentaenoic acid‐ethyl ester (EPA‐E). But direct cytotoxic action of macrophage on target cells (YAC‐1) was unchanged by the ingestion of EPA‐E. In addition, the depressed enhancing activity of EPA‐enriched macrophages (EPA macrophages) was partially, but significantly, restored by the addition of either 10 ‐10 M leukotriene B 4 (LTB 4 ) or 10 ‐12 M 5‐hydroperoxyeicosatetraenoic acid (5‐HPETE). The partial restoring effect of 10 ‐10 M LTB 4 was further significantly enhanced by the addition of 5‐HPETE. In contrast 5‐hydroxyeicosatetraenoic acid (5‐HETE) had no modulatory effect on the depressed activity of EPA macrophages. LTB 4 ,5‐HPETE, and 5‐HETE had no direct effect on NK cell activity itself. Significantly less amounts of LTB 4 and 5‐HETE were produced in EPA macrophages as compared with that in control macrophages. The present study indicates that macrophage modulatory action on NK cells can be partly mediated by LTB 4 and 5‐HPETE, which are produced in macrophages.