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In Vitro Modulating Effect of Human Very‐Low‐Density Lipoproteins on Human Polymorphonuclear Leukocyte Oxidative Metabolism and Migration
Author(s) -
Idohou N.,
Couderc R.,
RochArveiller M.,
Bonneau C.,
Giroud J.P.,
Raichvarg D.
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.50.1.1
Subject(s) - very low density lipoprotein , zymosan , superoxide , endocrinology , medicine , biology , in vitro , oxidative phosphorylation , stimulation , metabolism , biochemistry , lipoprotein , cholesterol , enzyme
In this work, the in vitro effects of very‐low‐density lipoproteins (VLDL) on human polymorphonuclear leukocyte (PMN) oxidative metabolism and migration were studied. VLDL stimulated PMN superoxide generation in absence of other stimulating agents. The effect of VLDL from normotriglyceridemic subjects was more marked than with VLDL from hypertriglyceridemia subjects. VLDL reduced in a dose‐dependent manner the luminol‐dependent chemiluminescence of PMN stimulated by phorbol myristate acetate (PMA) and, to a lesser degree, by opsonized zymosan. This effect was observed with VLDL concentrations found in healthy and hypertriglyceridemic patients. Superoxide anion generation was also reduced by preincubation of PMN with VLDL before stimulation with PMA but not opsonized zymosan. VLDL were not cytotoxic for PMN. The above effects appear to be an intrinsic property of VLDL and might lead to reduced PMN‐mediated non‐specific host defences in hypertriglyceridemic subjects.