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Recombinant Interleukin‐4 Enhances the Chemiluminescent Oxidative Burst of Murine Peritoneal Macrophages
Author(s) -
Tan Henkie P.,
NehlsenCannarella Sandra L.,
Garberoglio Carlos A.,
Tosk Jeffrey M.
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.49.6.587
Subject(s) - respiratory burst , monoclonal antibody , biology , recombinant dna , oxidative stress , chemiluminescence , macrophage , microbiology and biotechnology , cytokine , stimulation , oxidative phosphorylation , cell culture , respiratory system , immunology , antibody , biochemistry , chemistry , endocrinology , in vitro , anatomy , genetics , gene , organic chemistry
We evaluated the effects of murine recombinant interleukin‐4 (rIL‐4) on murine peritoneal macrophages. We showed a marked, dose‐dependent stimulation of respiratory oxidative burst by IL‐4 in peptone‐elicited murine peritoneal macrophages. This effect was abolished by a neutralizing monoclonal antibody (mAb) to rlL‐4 confirming that the enhanced chemiluminescence was due to IL‐4. In contrast, rlL‐4 depressed the respiratory oxidative burst of a transformed murine macrophage cell line, J774, in a dose‐dependent mAb‐reversible manner.