Premium
Restoration of Prostaglandin Releasing Macrophage Populations in Lethally Irradiated Mice With Spleen Cells From Bone Marrow‐Depleted Donors
Author(s) -
Shibata Yoshimi,
Volkman Alvin
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.49.4.397
Subject(s) - spleen , biology , bone marrow , macrophage , immunology , microbiology and biotechnology , biochemistry , in vitro
Previous studies in mice severely depleted of bone marrow cells by 89 Sr showed persistent monocytopenia and impaired expression of prostaglandin E 2 ‐releasing splenic macrophages (PGSM) despite the occurrence in the spleen of more than 10‐fold increases in pluripotential stem cells and Mo colony‐forming cells. To determine whether the observed deficits were due to a lack of precursors of blood monocytes and PGSM in the spleens of 89 Sr‐treated mice, radiation chimeras were established by iv infusion of 2 × 10 6 spleen cells from 89 Sr donor CBA/J or semisyngeneic B6CB F 1 hybrid mice into lethally γ‐irradiated CBA/J recipients. Blood monocyte levels were greater than normal by day 14 and PGSM induced by Corynebacterium parvum were demonstrated by day 28. These restored Mø populations expressed the donor haplotype detected in vitro with haplotypespecific monoclonal anti‐H‐2K plus complement. “Sr treatment of the chimeras resulted in profound depletion of monocytes and PGSM. The data indicate that the spleen of the 89 Sr‐treated mouse, which is an ineffective source of circulating monocytes and PGSM, contains cells which can generate both of these populations following infusion into lethally irradiated recipients. Since the bone marrow of such recipients was capable of being repopulated, the aggregate observations suggest that functional bone marrow is obligatory for the generation of blood monocytes and PGSM populations.