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Effect of Soluble Aminated β‐1,3‐D‐Polyglucose on Human Monocytes: Stimulation of Cytokine and Prostaglandin E 2 Production but Not Antigen‐Presenting Function
Author(s) -
Doita Minoru,
Rasmussen LillTove,
Seljelid Rolf,
Lipsky Peter E.
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.49.4.342
Subject(s) - prostaglandin e2 , biology , tumor necrosis factor alpha , cytokine , proinflammatory cytokine , immune system , stimulation , monocyte , prostaglandin e , antigen , secretion , macrophage activating factor , intracellular , microbiology and biotechnology , immunology , endocrinology , lymphokine , inflammation
Glucans are insoluble polymers of β‐1,3‐linked glucose derived from yeast cell walls that effectively activate macrophages. Recently, aminated derivatives of β‐1,3‐D‐polyglucose have been developed that are soluble but also activate murine macrophages. The current studies were undertaken to determine whether soluble aminated β‐1,3‐D‐polyglucose (AG) would also stimulate human monocytes. The AG employed contained <2 ng endotoxin/mg. AG induced the production of intracellular, membrane‐associated, and secreted forms of interleukin 1 (IL1) in a dose‐dependent manner, with 50 µg/ml yielding maximal responses. AG also induced tumor necrosis factor‐α (TNFα) secretion by human monocytes. Prostaglandin E 2 (PGE 2 ) production was also stimulated in a concentration‐dependent manner. Quantitatively, optimal stimulatory concentrations of AG were comparable to endotoxin in the capacity to induce production of these various mediators. In contrast to its capacity to induce production of IL1, TNFα, and PGE 2 , AG did not stimulate monocytes to become more effective antigen presenting cells. These results indicate that AG is a potent inducer of proinflammatory mediators from human monocytes but does not enhance their capacity to initiate immune responses.