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The Deficient Migration In Vitro of Lung Macrophages From Newborn Rats Normalizes After Three Days of Maturation In Situ
Author(s) -
Kurland Geoffrey,
Dauber James H.
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.49.2.211
Subject(s) - biology , in vitro , lung , motility , medicine , endocrinology , cell migration , signal transduction , phagocytosis , macrophage , andrology , immunology , microbiology and biotechnology , biochemistry
Because there are functional deficiencies in neonatal phagocytes, we investigated age‐dependent differences in the directed migration of rat pulmonary macrophages (PM). Directed migration of PMs from newborn (<24 h old) rats towards partially purified rat C5a was lower than that of PMs from adults, but reached adult levels by the third post‐natal day. Calculation of the dose of C5a resulting in a half‐maximal migratory response (ED‐50) revealed that the ED‐50s for all age groups studied were similar. These results suggest that responding PMs at all times after birth have comparable sensitivity to C5a, but that a lower percentage of newborn PMs migrate toward this factor because of defects in either signal transduction or cell motility.