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Stimulation of a Histone H4 Protein Kinase in Triton X‐100 Lysates of Rabbit Peritoneal Neutrophils Pretreated With Chemotactic Factors: Effect of Leukotriene B 4 and Cytochalasin B
Author(s) -
Huang ChiKuang,
Laramee Gary R.
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.49.2.158
Subject(s) - biology , chemotaxis , stimulation , leukotriene b4 , cytochalasin b , microbiology and biotechnology , protein kinase c , protein kinase a , kinase , immunology , biochemistry , endocrinology , receptor , in vitro , inflammation
The effects of leukotriene B 4 on the stimulation of a histone H4 kinase activity in rabbit peritoneal neutrophils have been studied. LTB 4 activated the kinase within 10 sec with the increasing activity decaying to near basal level after 20 sec of stimulation. The effect was not inhibited by removal of extracellular calcium or loading the cells with quin‐2/AM. Pretreatment of the cells with cytochalasin B greatly enhanced and prolonged the activation of the kinase. Pretreatment of the cells with LTB 4 or fMet‐Leu‐Phe inhibited the effect of the later addition of LTB 4 . Since similar characteristics of LTB 4 and cytochalasin B have also been observed in LTB 4 ‐stimulated responses such as secretion and superoxide generation, the results suggest a potential role of H4 kinase in LTB 4 ‐stimulated responses.