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Priming and Stimulation of Bovine Neutrophils by Recombinant Human interleukin‐1 Alpha and Tumor Necrosis Factor Alpha
Author(s) -
Sample Allen K.,
Czuprynski Charles J.
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.49.2.107
Subject(s) - monokine , zymosan , respiratory burst , stimulation , tumor necrosis factor alpha , recombinant dna , biology , microbiology and biotechnology , myeloperoxidase , superoxide , chemistry , interleukin , biochemistry , immunology , endocrinology , in vitro , cytokine , inflammation , enzyme , gene
We have examined the in vitro effects of two recombinant human monokines, interleukin‐1 alpha (rHulL‐1 α ) and tumor necrosis factor alpha (rHuTNF α ), on bovine neutrophil functions. Both rHulL‐1 α (10 to 1,000 ng/ml) and rHuTNF α (5 to 50 ng/ml) directly stimulated the oxidative burst of bovine neutrophils as measured by Luminol‐dependent chemiluminescence, superoxide anion generation, and hydrogen peroxide production. In addition, both rHulL‐1 α (1 to 1,000 ng/ml) and rHuTNF α (0.5 to 50 ng/ml) primed bovine neutrophils for an enhanced oxidative burst to subsequent stimulation with opsonized zymosan. Neutrophils pretreated with either monokine exhibited an earlier, as well as stronger, zymosan‐stimulated Luminol‐dependent chemiluminescence response, as compared to untreated neutrophils. Exposure of bovine neutrophils to combinations of suboptimal doses of rHulL‐1 α (10 and 100 ng/ml) and rHuTNF α (0.5 and 5 ng/ml) resulted in a synergistic stimulation of Luminol‐dependent chemiluminescence, whereas, no synergism was observed when using optimal doses of each monokine. Pre‐incubation of bovine neutrophils with an optimal concentration of recombinant bovine interferon gamma (100 U/ml), and either rHulL‐1 α or rHuTNF α further augmented the maximal oxidative response of neutrophils stimulated with opsonized zymosan. Bovine neutrophils released both primary and secondary granules in response to rHulL‐1 α and rHuTNF α , and also exhibited enhanced adherence in the presence of either monokine.